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Thursday, July 23, 2020 | History

2 edition of Attachment and outgrowth of swine blastocysts in vitro found in the catalog.

Attachment and outgrowth of swine blastocysts in vitro

Shannon Jack Shaffer

Attachment and outgrowth of swine blastocysts in vitro

by Shannon Jack Shaffer

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  • 33 Currently reading

Published .
Written in English

    Subjects:
  • Blastocyst.

  • Edition Notes

    Statementby Shannon Jack Shaffer.
    The Physical Object
    Paginationviii, 49 l. :
    Number of Pages49
    ID Numbers
    Open LibraryOL16720255M

    Hatching, attachment, and trophoblast outgrowth of mouse embryos in vitro were examined as a model for implantation. Mouse embryos attached and grew out on glass cover slips that were partially covered with cultured mouse cells (L cells, liver cells, transformed JLS-V11 cells, and teratocarcinoma cells).   Abstract. BACKGROUND: This study was designed to examine the cytotoxic effect of retinoic acid on the blastocyst stage of mouse embryos and on subsequent early postimplantation embryo development S AND RESULTS: Mouse blastocysts were exposed for 24 h to doses of 0, µmol/l and 10 µmol/l all‐trans retinoic acid and .

      Tao, T. & Niemann, H. Cellular characterization of blastocysts derived from rabbit 4-, 8- and cell embryos and isolated blastomeres cultured in vitro. Hum. Reprod. 15, . Mouse blastocysts were studied using this in vitro model to determine whether entactin was capable of mediating trophoblast invasive activity. Although entactin has never been shown to promote cell migration, we report here that recombinant entactin supported blastocyst outgrowth in a dose-dependent manner, with a maximal effect at

    In this study, we examined the cytotoxic effects of curcumin, the yellow pigment of Curcuma longa, on the blastocyst stage of mouse embryos, subsequent embryonic attachment, and outgrowth in vitro and in vivo implantation by embryo transfer. Mouse blastocysts were incubated in medium with or without curcumin (6, 12 or 24 μM) for 24 h.   Embryo implantation into the maternal uterus is a decisive step for successful mammalian pregnancy. Osteopontin (OPN) is a member of the small integrin-binding ligand N-linked glycoprotein family and participates in cell adhesion and invasion. In this study, we showed that Opn mRNA levels are up-regulated in the mouse uterus on day 4 and at the implantation .


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Attachment and outgrowth of swine blastocysts in vitro by Shannon Jack Shaffer Download PDF EPUB FB2

Swine Blastocyst Attachment and Size at Attachment to Cellular and Non-cellular Substrata In Vitro Number of Blastocyst Diameter Substrata Number of Blastocysts 24 hr Prior to Blastocysts Attached Attachment SO X (Pm) MEM 32 7a Buf 35 21b e Btes 28 lc d cMEM 27 9a a,b,c,d,eValues with different Cited by: The process of mammalian implantation has been investigated using an in vitro model system wherein the trophoblast cells of mouse blastocysts attach to and outgrow on tissue culture plates containing a complex medium.

We now report that two extracellular matrix glycoproteins, fibronectin and laminin, when individually precoated on tissue culture plates promoted in vitro attachment Cited by: The proportions of hatched blastocysts showing adhesion or outgrowth were used to estimate the implantation capacity of blastocysts in vitro.

SCNT and Subsequent Embryo Cultures Vitrification of failed-to-be-fertilized oocytes was performed using hemi-straws with a vitrification kit (CooperSurgical Inc., Trumbull, CT) as described [22].Cited by: Shaffer and Wright ()/J Anim Sci Swine Attachment and trophoblastic outgrowth of swine blastocysts in vitro Atienza-Samols and Sherman ()/Dev Biol Mouse Outgrowth.

Protein secretion from mouse blastocysts undergoing attachment and trophoblast outgrowth in vitro was assessed. When Day 5 blastocysts were cultured in. Protein secretion from mouse blastocysts undergoing attachment and trophoblast outgrowth in vitro was assessed. When Day 5 blastocysts were cultured in serum-containing medium, secretion of several 'attachment-associated' proteins (PAS) was initiated within 24 h, coincident with attachment and outgrowth.

Those proteins characteristic of the pre-attachment. Books; JCB Journal of Cell Mouse-hatched blastocysts cultured in vitro will attach and form outgrowths of trophoblast cells on appropriate substrates, providing a model for implantation. Attachment and trophoblast outgrowth on these substrates can be inhibited by addition to the culture medium of an antibody, anti-ECMr (anti.

Part of the Methods in Molecular Medicine™ book series (MIMM H. A., and Lennarz, W. () Fibronectin and laminin promote in vitro attachment and outgrowth of mouse blastocysts. R., Kaplan, H. A., Mover, H., and Lennarz, W. () The effect of hexapeptides on attachment and outgrowth of mouse blastocysts cultured in vitro.

In this study, we examined the cytotoxic effects of curcumin, the yellow pigment of Curcuma longa, on the blastocyst stage of mouse embryos, subsequent embryonic attachment, and outgrowth in vitro and in vivo implantation by embryo transfer. Mouse blastocysts were incubated in medium with or without curcumin (6, 12 or 24 μM) for 24 h.

Cell proliferation and. During this time, essential changes in the endometrium take place in preparation for attachment of the blastocyst and implantation. Early embryonic loss is an economic problem of global proportions in animal husbandry, where, in pigs and cattle for example, some 30% of all fertilizations fail to result in a pregnancy.

Ochratoxin A (OTA), a mycotoxin found in many foods worldwide, causes nephrotoxicity, hepatotoxicity, and immunotoxicity, both in vitro and in vivo.

In the present study, we explored the cytotoxic effects exerted by OTA on the blastocyst stage of mouse embryos, on subsequent embryonic attachment, on outgrowth in vitro, and following in vivo implantation via embryo transfer. Mouse blastocysts. J Anim Sci. Jun;46(6) Attachment and trophoblastic outgrowth of swine blastocysts in vitro.

Shaffer SJ, Wright RW Jr. PMID. B, Mouse blastocysts were incubated with or without ENN B1 (1, 5, or 10 μM) for 24 hours followed by culture in fibronectin‐coated dishes for 7 days.

According to morphological assessment, the status of outgrowth of blastocysts in cultured dishes was classified as attachment only, ICM+, ICM++, and ICM+++, as described in Section 2. Effect of the novel system on the attachment and outgrowth of blastocysts.

As it is difficult to isolate ICM cells from intact porcine blastocysts produced in vitro, whole blastocysts were plated directly onto mouse embryonic ing plating, the attachment rates were higher in blastocysts produced by the novel system (±% in PA and ±% in.

The peri-implantation development involves zona escape (hatching) of blastocysts and their attachment and proliferation. These events are difficult to studyin vivo, so in this study hamster 8-cell embryos were cultured through the hatched and attached blastocyst stages using different formulations of hamster embryo culture medium (HECM) Supplementation of succinate.

Morris, J.E., Potter, S.W. () An in vitro model for studying interactions between mouse trophoblast and uterine epithelial cells. A brief review of in vitro systems and observations on cell-surface changes during blastocyst attachment.

Trophoblast Res.4, 51– Google Scholar. Abstract. Hatching, attachment, and trophoblast outgrowth of mouse embryos in vitro were examined as a model for implantation.

Mouse embryos attached and grew out on glass cover slips that were partially covered with cultured mouse cells (L cells, liver cells, transformed JLS-V11 cells, and teratocarcinoma cells). We attempted to isolate ES cell lines using inner cell masses from high-quality cloned porcine blastocysts.

After being seeded onto feeders, embryos had better (P attachment, outgrowth formation and primary colonization in both 2× and 3× aggregated cloned embryos (, and% vs.and %, respectively) compared to the non. Complete zona pellucida removal from vitrified-warmed human blastocysts facilitates earlier in-vitro attachment and outgrowth.

Reprod Biomed Online. ; View in Article. In addition, in‐vitro studies have shown that heparin‐binding epidermal growth factor (HB‐EGF) mediates the adhesion of mouse blastocysts (Raab et al., ), and fibronectin and its receptors mediate trophoblast outgrowth in models of blastocyst adhesion (Yelian et al., ).

The blastocyst is a structure formed in the early development of possesses an inner cell mass (ICM) which subsequently forms the outer layer of the blastocyst consists of cells collectively called the layer surrounds the inner cell mass and a fluid-filled cavity known as the trophoblast gives rise to the placenta.

2-Bromopropane (2-BP), an alternative to ozone-depleting solvents, is used as a cleaning solvent. Here, we examined the cytotoxic effects of 2-bromopropane (2-BP) on mouse embryos at the blastocyst stage, subsequent embryonic attachment and outgrowth in vitro, and in vivo implantation via embryo transfer.

Mouse blastocysts were incubated in medium with or .We examined the cytotoxic effects of dillapiole, a phenylpropanoid with antileishmanial, anti-inflammatory, antifungal, and acaricidal activities, on the blastocyst stage of mouse embryos, subsequent embryonic attachment and outgrowth in vitro, and in vivo implantation via embryo transfer.

Blastocysts treated with –10 μM dillapiole exhibited a significant increase in .